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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1995-12-12
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D14157,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L15453,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L27745,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M23919,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M57682,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M64373,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M67515,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M67516,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M76558,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M83566,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M92905,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M94172,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M94173,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X57476,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X57477,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X67855,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X67856
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pubmed:abstractText |
The identity of alpha 1 subunits from voltage operated Ca2+ channels was determined in the rat/mouse mesencephalon x N18TG2 hybridoma cell line MES23.5, by sequence analysis of reverse transcription-polymerase chain reaction products and antagonist binding. Sequences were derived from the L-(alpha 1D), Q-(alpha 1A) and omega-conotoxin GVIA sensitive N-type (alpha 1B) Ca2+ channel alpha 1 subunits. The amplified fragments, which are homologous to the region between domain III and IV of known alpha 1 subunits, reveal splice variation in the L- and Q-type alpha 1 subunit of MES23.5 cells. The transcripts of alpha 1 subunits in these cells were quantified by RNAase protection assay. The data show the existence of different Ca2+ channel types in a single cell line and may reflect multiple functions of voltage operated Ca2+ channels during growth, differentiation and transmitter release.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0306-4522
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
479-85
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:7477958-Amino Acid Sequence,
pubmed-meshheading:7477958-Animals,
pubmed-meshheading:7477958-Base Sequence,
pubmed-meshheading:7477958-Brain Neoplasms,
pubmed-meshheading:7477958-Calcium Channel Blockers,
pubmed-meshheading:7477958-Calcium Channels,
pubmed-meshheading:7477958-Carps,
pubmed-meshheading:7477958-Cloning, Molecular,
pubmed-meshheading:7477958-Electrophysiology,
pubmed-meshheading:7477958-Humans,
pubmed-meshheading:7477958-Hybrid Cells,
pubmed-meshheading:7477958-Ion Channel Gating,
pubmed-meshheading:7477958-Mesencephalon,
pubmed-meshheading:7477958-Molecular Sequence Data,
pubmed-meshheading:7477958-Neuroblastoma,
pubmed-meshheading:7477958-Polymerase Chain Reaction,
pubmed-meshheading:7477958-RNA, Messenger,
pubmed-meshheading:7477958-Rabbits,
pubmed-meshheading:7477958-Ribonucleases,
pubmed-meshheading:7477958-Species Specificity,
pubmed-meshheading:7477958-Tumor Cells, Cultured
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pubmed:year |
1995
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pubmed:articleTitle |
Alpha-1 subunits of voltage gated Ca2+ channels in the mesencephalon x neuroblastoma hybrid cell line MES23.5.
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pubmed:affiliation |
Department of Neurology, Baylor College of Medicine, Houston, TX 77030, USA.
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pubmed:publicationType |
Journal Article
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