Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1995-11-30
|
| pubmed:abstractText |
Human T-lymphotropic virus type 1 (HTLV-I) is a retrovirus associated with adult T-cell leukemia/lymphoma, and the virus infection constitutes a growing public health problem. In a continuing effort to engineer conformationally dependent HTLV-I epitopes that elicit a protective immune response, we have examined the role and functional importance of carbohydrate moieties in specific immune recognition and antibody responses. There have been several reports of the importance of N-linked virus glycosylation in the formation of neutralizing antibodies. Residues 230-257 is predicted to encode two beta-turn/loop regions at 240-244 (LYGPN), 248-257 (VPSSSSTPL) and a glycosylation site at N-244 (NVS). We have successfully engineered and synthesized the 233-253 sequence of gp46 of HTLV-1 with and without GlcNAC at Asn244. Circular dichroism spectroscopy and proton NMR showed the presence of beta-turn conformation in both peptide constructs. Chimeras of the glycosylated and non-glycosylated epitope with promiscuous T-cell epitope were synthesized and shown to elicit high titered antibodies in rabbits specific for the immunogen (SC1MVF and SC2MVF) and the B cell epitope 233-253. Additionally, antibodies to the glycosylated form of the peptide recognized the HTLV-I envelope precursor in radioimmunoassay precipitation assay and react with HTLV-I whole virus preparations in ELISA.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, env,
http://linkedlifedata.com/resource/pubmed/chemical/Glycopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Retroviridae Proteins, Oncogenic,
http://linkedlifedata.com/resource/pubmed/chemical/gp46 protein, Human T-cell...
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0887-6924
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
9 Suppl 1
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
S133-8
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7475306-Adult,
pubmed-meshheading:7475306-Amino Acid Sequence,
pubmed-meshheading:7475306-Animals,
pubmed-meshheading:7475306-Antibodies,
pubmed-meshheading:7475306-Antibody Formation,
pubmed-meshheading:7475306-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:7475306-Epitopes,
pubmed-meshheading:7475306-Gene Products, env,
pubmed-meshheading:7475306-Genes, env,
pubmed-meshheading:7475306-Glycopeptides,
pubmed-meshheading:7475306-Glycosylation,
pubmed-meshheading:7475306-Human T-lymphotropic virus 1,
pubmed-meshheading:7475306-Humans,
pubmed-meshheading:7475306-Leukemia, T-Cell,
pubmed-meshheading:7475306-Magnetic Resonance Spectroscopy,
pubmed-meshheading:7475306-Molecular Sequence Data,
pubmed-meshheading:7475306-Peptide Fragments,
pubmed-meshheading:7475306-Rabbits,
pubmed-meshheading:7475306-Retroviridae Proteins, Oncogenic
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Glycosylation-dependent peptide antigenic determinants of env gp46 HTLV-1.
|
| pubmed:affiliation |
Department of Obstetrics & Gynecology, College of Medicine, Ohio State University, Columbus 43210, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|