Linked Life Data

7457627

Source:http://linkedlifedata.com/resource/pubmed/id/7457627

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-3-24
pubmed:abstractText
Amino acid, [3H]lysine, incorporation was examined with autoradiographic techniques in four nuclei of the medial hypothalamus during four consecutive 6-h periods. Rats which had previously been maintained under 12:12 light-dark conditions were kept in constant darkness during the day of [3H]lysine administration to prevent any direct influence of light on hypothalamic activity. Forty-five minutes after intraperitoneal injection of the tritiated amino acid, animals were killed. After autoradiography processing, grain counting revealed that the suprachiasmatic and magnocellular paraventricular nuclei showed a statistically significant circadian variation in the number of grains over neural perikarya. A time-of-day effect was also found in the grain count over neuropil of the arcuate, ventromedial, and paraventricular nuclei. A corresponding behavioral study found that feeding continued to occur primarily during the 12 h of night, even with lights out for 24 h. These data suggest an endogenous circadian variation in one or more factors that influence amino acid uptake and protein synthesis in some hypothalamic neurons. When [3H]lysine uptake was analyzed in terms of grains/100 micrometer 2 of cell area, the arcuate and paraventricular nuclei showed a significantly greater (double) uptake of [3H]lysine than did the suprachiasmatic or ventromedial nuclei. Four times more grains were counted over somata than over an equal area of cell-free neuropil.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
240
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
R16-22
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Amino acid incorporation in medial hypothalamic nuclei: circadian, perikarya, and neuropil variations.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.