Linked Life Data

7452092

Source:http://linkedlifedata.com/resource/pubmed/id/7452092

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1981-3-24
pubmed:abstractText
A method is described in which the interaction between thrombin and platelet proteins can be studied directly. Solubilized platelet proteins were examined by crossed immunoelectrophoresis against polyspecific antiplatelet antibodies, and the interaction with thrombin observed by insertion of an intermediate gel containing thrombin coupled to Sepharose 4-B. Four immunoprecipitates were absent or showed an altered position compared to that of the control containing regular Sepharose 4-B in the intermediate gel. These were No. 1 (representing platelet factor 4), No. 13 (glycocalicin-related protein, probably GP lb), No. 19 (factor XIII), and No. 2a which has not yet been identified. Immunoprecipitates No. 16, representing a complex of GP IIb and GP IIIa, and No. 6 (albumin) as well as the other immunoprecipitates seen in the control pattern were unaffected by the presence of immobilized thrombin in the intermediate gel. The interaction of glycocalicin-related protein as well as purified glycocalicin with thrombin was confirmed by use of a monospecific antiserum. It is concluded that crossed immunoelectrophoresis using immobilized thrombin in an intermediate gel represents a new and useful approach to the investigation of the interaction between thrombin and platelet proteins. This procedure may also be extended to many other interactions between agents and cell proteins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-2143
pubmed:author
pubmed:issnType
Print
pubmed:volume
97
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
213-20
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Crossed immunoelectrophoresis using immobilized thrombin in intermediate gel. A method for demonstration of thrombin-binding platelet proteins.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't