Linked Life Data

7449736

Source:http://linkedlifedata.com/resource/pubmed/id/7449736

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1981-3-27
pubmed:abstractText
In the present studies, we have evaluated the effects of fasting, variations in thyroid state, and interactions thereof on the kinetic properties of the microsomal enzyme in rat liver that generates T3 from T4. The formation of T3 and T4 (T3-neogenesis) in preparations of rat liver microsomes enriched with 10 mM dithiothreitol was monitored by assessing the formation of [125I]T3 from [125I]T4 in the presence of various concentrations of stable T4. T3-neogenesis in this system was a saturable process that obeyed the laws of first order enzyme kinetics. The effects of diverse in vivo manipulations on the kinetics of T3-neogenesis were assessed. The Vmax was markedly decreased in preparations from thyroidectomized animals, being, respectively, partially and completely restored to normal with daily maintenance therapy with T4, (0.5 and 1.5 microgram/100 g BW) and markedly increased after the administration of supraphysiological replacement doses of T4 (5.0 microgram/100 g . day). Km was not affected by alterations in thyroid state. In experiments concerning the effects of fasting, cognizance was taken of the fact that hypothyroidism regularly evolves as a consequence of fasting. The Vmax was decreased markedly, and Km was slightly decreased in preparations from intact rats fasted 72 h but not given physiological replacement with T4 or T3. In contrast, Vmax and Km were unchanged in hepatic microsomes from intact rats fasted 72 h but given daily parenteral replacement with T4 (1.5 microgram/100 g) or T3 (0.5 microgram/100 g). These data demonstrate that hypothyroidism resulting from either thyroidectomy or fasting produces decreased intrinsic activity of the microsomal T4 5'-monodeiodinase. In contrast, fasting without concurrent hypothyroidism does not influence the intrinsic activity of the hepatic T3-neogenetic enzyme. (Endocrinology 108: 472, 1981)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
108
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
472-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
The influence of fasting and the thyroid state on the activity of thyroxine 5'-monodeiodinase in rat liver: a kinetic analysis of microsomal formation of triiodothyronine from thyroxine.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.