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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
1981-3-24
|
| pubmed:abstractText |
Cell suspension of the rat brain was separated into fractions of neuronal and glial cells (FNC, FGC) during 7-10 minutes. The suspension was filtered in the column through a series of filters with 400-600, 200 (FNC), 132 and 100 (FGC) mu pores. The purity of the fractions obtained was analyzed by light microscopy. According to a single cell counting the purity of FNC and FGC contituted 60 and 90%, respectively. The respective figures were 97 and 90% as shown by cell weight comparison. The biochemical and morphological findings indicate a good viability of isolated cells. It has been established that of total rat brain protein (108 mg per 1 g crude tissue), the proteins of nerve cells account for about 30%. The mass ratio of neurons to glial cells is 2.5:1. This fact should be taken into consideration during studies on the functional interactions between neurons and glial cells.
|
| pubmed:language |
rus
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0365-9615
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
90
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
630-2
|
| pubmed:dateRevised |
2008-10-8
|
| pubmed:meshHeading | |
| pubmed:year |
1980
|
| pubmed:articleTitle |
[Rapid method of obtaining enriched fractions of rat brain neuronal and glial cells].
|
| pubmed:publicationType |
Journal Article,
English Abstract
|