Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1978-2-23
|
| pubmed:abstractText |
Two maxima of optic density were observed at zones of gravity 1.27 g/ml and 1.15-1.16 g/ml by sedimentation equilibrium in sucrose gradient of cultural fluid, obtained from the transplantable cells of the HEP-2 strain and concentrated by ultracentrifugation. These fractions thus isolated were tested for presence of RNA- and DNA-dependent DNA-polymerase. The structures with the density of 1.15-1.16 g/ml were identified with the oncornaviruses on the basis of characteristics flotating density, presence of RNA-dependent DNA-polymerase. Analyses of products of RNA- and DNA-dependent polymerases reaction, flotating density of oncornaviral nucleotides in sucrose and CsCl gradients are presented. The optimal conditions for reverse-transcriptase reaction of virions of D type viruses are characterized.
|
| pubmed:language |
rus
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0042-8809
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
824-9
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:articleTitle |
[Further study of spontaneous virus production using transplantable HEp-2 cells as a model].
|
| pubmed:publicationType |
Journal Article,
English Abstract
|