Linked Life Data

7410853

Source:http://linkedlifedata.com/resource/pubmed/id/7410853

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1980-11-25
pubmed:abstractText
Thoracic duct lymph contains a factor that inhibits in vitro adhesion between lymphocytes and high endothelial cells. Crude inhibitory factor was isolated from lymph by (NH4)2SO4 precipitation and partially purified by using an immunoabsorbant column of rabbit anti peak I Ig coupled to Sepharose 4B. Antibody affinity chromatography separated the HEV binding inhibitory factor from the bulk of protein in the crude preparation; activity was enriched 50-fold. The results show that the effect of the factor was exerted on high endothelial cells; both glutaraldehyde-fixed HEV and unfixed mouse HEV were susceptible to the action of this material. In contrast, the HEV binding properties of lymphocytes were unaffected by the factor. Inhibitory activity was destroyed by treatment with trypsin or exposure to 100 degrees C but was unaffected by incubation at 56 or 70 degrees C for 30 min. In addition, the factor bound to lentil lectin and was eluted with alpha-methyl-D-mannoside. Together these findings indicate that the HEV binding inhibitory factor is a glycoprotein.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:volume
125
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1770-74
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Lymphocyte recognition of lymph node high endothelium. II. Characterization of an in vitro inhibitory factor isolated by antibody affinity chromatography.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.