Linked Life Data

7408795

Source:http://linkedlifedata.com/resource/pubmed/id/7408795

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1980-11-20
pubmed:abstractText
Tracheal slices incubated with radioactive precursors in tissue culture medium secrete labeled mucus glycoproteins into the culture medium. We have used an in vivtro approach, a combined method utilizing exposure to pneumotoxins in vivo coupled with quantitation of mucus secretion rates in vitro, to study the effects of inhaled pollutants on mucus biosynthesis by rat airways. In addition, we have purified the mucus glycoproteins secreted by rat tracheal explants in order to determine putative structural changes that might by the basis for the observed augmented secretion rates after exposure of rats to H2SO4 aerosols in combination with high ambient levels of ozone. After digestion with papain, mucus glycoproteins secreted by tracheal explants may be separated into five fractions by ion-exchange chromatography, with recovery in high yield, on columns of DEAE-cellulose. Each of these five fractions, one neutral and four acidic, migrates as a single unique spot upon cellulose acetate electrophoresis at pH values of 8.6 and 1.2. The neutral fraction, which is labeled with [3H] glucosamine, does not contain radioactivity when Na2 35SO4 is used as the precursor. Acidic fractions I-IV are all labeled with either 3H-glucosamine or Na2 35SO4 as precursor. Acidic fraction II contains sialic acid as the terminal sugar on its oligosaccharide side chains, based upon its chromatographic behavior on columns of wheat-germ agglutinin-Agarose. Treatment of this fraction with neuraminidase shifts its elution position in the gradient to a lower salt concentration, coincident with acidic fraction I. After removal of terminal sialic acid residues with either neuraminidase or low pH treatment, the resultant terminal sugar on the oligosaccharide side chains is fucose. These results are identical with those observed with mucus glycoproteins secreted by cultured human tracheal explants and purified by these same techniques.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7408795-125590, http://linkedlifedata.com/resource/pubmed/commentcorrection/7408795-1275505, http://linkedlifedata.com/resource/pubmed/commentcorrection/7408795-14200692, http://linkedlifedata.com/resource/pubmed/commentcorrection/7408795-4705475, http://linkedlifedata.com/resource/pubmed/commentcorrection/7408795-4786535, http://linkedlifedata.com/resource/pubmed/commentcorrection/7408795-4988950, http://linkedlifedata.com/resource/pubmed/commentcorrection/7408795-5011666, http://linkedlifedata.com/resource/pubmed/commentcorrection/7408795-5541467, http://linkedlifedata.com/resource/pubmed/commentcorrection/7408795-621431, http://linkedlifedata.com/resource/pubmed/commentcorrection/7408795-921053, http://linkedlifedata.com/resource/pubmed/commentcorrection/7408795-999298
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0091-6765
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
131-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Mucus glycoprotein secretion by tracheal explants: effects of pollutants.
pubmed:publicationType
Journal Article