Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1980-9-26
pubmed:abstractText
Mitochondria isolated from rat liver after a short-term perfusion with the alpha-adrenergic agonist phenylephrine or with glucagon exhibited enhanced rates of uptake of Ca2+ and prolonged retention of Ca2+ in the presence of 4mm-P(i). The effect of Ca2+ retention was apparent after perfusion with phenylephrine for only 1min and was maximal after 7min of treatment. The changes induced by glucagon, although similar, were less rapid. Adrenaline caused similar changes to phenylephrine and its effects were blocked by the alpha-adrenergic antagonist phenoxybenzamine, but not by the beta-antagonist propranolol. The Ca2+ content of the isolated mitochondria decreased by 30% 1min after the onset of perfusion with phenylephrine; by 6min it had begun to return to the original value which was reached at 10min. A similar loss in calcium content was induced by glucagon but the changes were not as great and occurred more slowly. Mitochondria from phenylephrine-treated livers exhibited decreased rates of Ca2+ efflux induced by addition of 2mm-EGTA, a 50% increase in the contents of ADP and total adenine nucleotides, a small increase in the transmembrane pH gradient, and a reduced rate of oxaloacetate-induced NADPH oxidation. This study thus shows that stimulation of liver by alpha-adrenergic agonists, like that by glucagon, induces within minutes a stable modification of mitochondria leading to alterations in the Ca2+-translocation cycle (increased Ca2+ uptake and retention) and alterations in mitochondrial energy-linked reactions.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-1212198, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-13376584, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-140067, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-14087334, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-196302, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-196940, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-210759, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-221257, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-240844, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-25436, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-27255, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-27509, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-323250, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-353809, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-357436, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-437092, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-4375957, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-4452361, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-457663, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-5776240, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-728072, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-747647, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-807125, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-8456, http://linkedlifedata.com/resource/pubmed/commentcorrection/7396872-8457
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
188
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
443-50
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Stable changes to calcium fluxes in mitochondria isolated from rat livers perfused with alpha-adrenergic agonists and with glucagon.
pubmed:publicationType
Journal Article, In Vitro