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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1979-3-29
|
| pubmed:abstractText |
The purification was begun with acetone precipitation of minced brain tissue with subsequent proteinase extraction with 0.2 M sodium formate buffer (pH 3.5), reprecipitation with acetone and dialysis. Chromatographic separation on Sephadex G-200, DEAE-Sephadex A-50 and CM-cellulose was carried out in that order. Upon ion-exchange chromatography multiple forms of acid proteinases emerged; two of them were obviously identical with cathepsin D (EC 3.4.23.5), and two of them exhibited properties of cathepsin B (EC 3.4.22.1).
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0302-4369
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
B32
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
727-32
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:735639-Animals,
pubmed-meshheading:735639-Brain,
pubmed-meshheading:735639-Cattle,
pubmed-meshheading:735639-Chromatography, DEAE-Cellulose,
pubmed-meshheading:735639-Chromatography, Gel,
pubmed-meshheading:735639-Chromatography, Ion Exchange,
pubmed-meshheading:735639-Endopeptidases
|
| pubmed:year |
1978
|
| pubmed:articleTitle |
Purification of acid proteinases from calf brain.
|
| pubmed:publicationType |
Journal Article
|