|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:dateCreated |
1982-3-22
|
|
pubmed:abstractText |
1. A two-micro-electrode voltage-clamp technique was applied to a study of the resting properties of mouse pancreatic acinar cell membranes and the action of acetylcholine (ACh). 2. The resting voltage-current relation was linear. The specific membrane resistance was calculated to be about 10 k omega cm2. This value was doubled after removal of Cl from the tissue bath superfusion solution. 3. At a holding potential equal to the spontaneous resting potential (about -35 mV) micro-ionophoretic ACh application evoked inward current. Reversal of the polarity of the ACh-evoked current occurred at about - 15 mV. 4. The voltage dependence of the ACh-evoked current displayed inward rectification. This inward rectification could not be accounted for by the constant field equation. 5. The dose-response curves for ACh-evoked inward current were compared in the same units with dose-response curves for ACh-evoked depolarization. Half-maximal depolarization was consistently obtained at a lower dose of ACh than half-maximal inward current. 6. During steady-state exposure of the pancreatic tissue segments to Cl-free sulphate solution the ACh reversal potential was about + 10 mV and the voltage-current relationship for the ACh-controlled channels showed inward rectification. Removal of external Na from the Cl-free solution virtually abolished ACh-evoked inward current. 7. The resting pancreatic acinar cell membrane is electrically inexcitable. The relative permeabilities of the major monovalent ions appears to be PC1/PNa/PK = 2/0.23/1. The ACh-evoked inward current is largely carried by Na. Dose-response curves for ACh-evoked depolarization and inward current in the same acinar units are different, in such a way that the depolarization response saturates at lower ACh concentrations than the current response.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-1081139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-1103635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-1142124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-13748311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-18128147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-224401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-309943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-360950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-37516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-4352766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-4363955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-4541341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-4543940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-4679700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-469765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-4702455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-5071933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-567789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-568772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-569159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-6105844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-625014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-68708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-6994596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-731453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-7463372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-894612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-894613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320904-943088
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0022-3751
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
318
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
57-71
|
|
pubmed:dateRevised |
2010-9-13
|
|
pubmed:meshHeading |
|
|
pubmed:year |
1981
|
|
pubmed:articleTitle |
Mouse pancreatic acinar cells: voltage-clamp study of acetylcholine-evoked membrane current.
|
|
pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|
