Linked Life Data

7299130

Source:http://linkedlifedata.com/resource/pubmed/id/7299130

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1982-1-28
pubmed:abstractText
C3H/HeN resident peritoneal macrophages in suspension culture supported continuous replication of L. tropica amastigotes; the total number of intracellular parasites increased 8- to 10-fold over 96 hr in culture. Lymphokine treatment of macrophages markedly affected intracellular replication of the Leishmania. Cultures treated with lymphokines before exposure to L. tropica were more resistant to infection, and 35% fewer cells contained intracellular amastigotes compared to medium-treated controls. Lymphokine-pretreated cells that became infected also inhibited the replication of intracellular amastigotes. Macrophage cultures treated with lymphokines after infection exhibited potent microbicidal activity; 75 to 80% of macrophages were free of intracellular parasites by 72 hr. Fractionation of lymphokine supernatants by Sephadex G-100 demonstrated 3 areas of activity for the induction of macrophage intracellular killing (130,000, 45,000, less than or equal to 10,000 daltons); one of these activity peaks (45,000-m.w. lymphokine(s)) also induced increased resistance to infection with L. tropica.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:volume
127
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2381-6
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Intracellular replication and lymphokine-induced destruction of Leishmania tropica in C3H/HeN mouse macrophages.
pubmed:publicationType
Journal Article