Linked Life Data

7261314

Source:http://linkedlifedata.com/resource/pubmed/id/7261314

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1981-10-29
pubmed:abstractText
A band that is strongly fluorescent and migrates electrophoretically with serum albumin is commonly found in electrophoretograms of sera from patients with chronic renal failure. We sought to determine whether the fluorescence originates from binding of certain still-unidentified metabolites or drugs, from an abnormal albumin species, or from some other protein entity. Molecular-exclusion column chromatography, polyacrylamide gel isoelectric focusing, and cellulose acetate electrophoresis, along with results of charcoal treatment and alcohol extraction, provided evidence that the fluorescence comes from fluorescent ligands tightly bound to albumin. The fluorescent intensity of the albumin fraction, isolated by molecular-exclusion chromatography, coincides with the albumin-associated fluorescence determined electrophoretically and with the intensities of the fluorescence emission spectrum for serum. A fluorescent species with an emission maximum of 415 +/- 5 nm, separated by thin-layer chromatography, appears to account for the increased serum fluorescence.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0009-9147
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
649-52
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Albumin-bound fluorescence in serum of patients with chronic renal failure.
pubmed:publicationType
Journal Article