Linked Life Data

7237555

Source:http://linkedlifedata.com/resource/pubmed/id/7237555

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1981-8-20
pubmed:abstractText
The major coat protein (gene 8 product) of bacteriophage M13 is an integral membrane protein during infection of host cells. It is synthesized as a larger precursor (procoat) with a leader sequence of 23 amino acids at its amino terminus. In vivo studies have shown that procoat only inserts into the host-cell plasma membrane after its synthesis is completed. We now demonstrate that procoat can post-translationally insert into inverted cytoplasmic membrane vesicles from E. coli and can be processed proteolytically to yield coat protein. Procoat changes from an assembly-competent substrate to an incompetent (denatured) form within minutes after its synthesis; much of the procoat that accumulates during an hour of in vitro synthesis is therefore denatured. These studies emphasize the importance of stringent criteria for the demonstration of obligate cotranslational assembly.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0092-8674
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
437-41
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Membrane assembly: posttranslational insertion of M13 procoat protein into E. coli membranes and its proteolytic conversion to coat protein in vitro.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't