Linked Life Data

7228861

Source:http://linkedlifedata.com/resource/pubmed/id/7228861

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1981-7-20
pubmed:abstractText
Interaction of four different asialotransferrins (human types 1, 2, and 3, and rabbit asialotransferrin) with purified plasma membranes from the rat liver was studied by using a direct binding assay. Binding of rabbit asialotransferrin, possessing a single biantenary glycan, was too weak to establish a complete binding curve, but the human asialotransferrins, possessing two glycan attachments, did yield binding data over a sufficiently wide range of concentrations for Scatchard plot analysis. At 22 degrees C, a quantity of plasma membrane equivalent to 1 mg of membrane protein bound comparable quantities (12.3-12.8 pmol) of the asialotransferrin types with an association constant of 1.5 X 10(6) M-1 for type 1, 1.4 X 10(7) M-1 for type 2, and 1.1 X 10(8) M-1 for type 3. At 4 degrees C, the number of binding sites and the association constants were reduced, more so for asialotransferrin types 2 and 3 than for type 1. At both temperatures, the shapes of the Scatchard plots for all three asialotransferrin types were similar: in the low range of bound asialoprotein (below 0.6-0.7 nM), each plot exhibited two to three convex peaks, tentatively identified as restricted domains of positive cooperativity; in the higher range, however, the plots were linear. The findings are consistent with the view that the binding sites involved in the binding of asialotransferrin are homogenous.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
256
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4929-33
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Binding of asialotransferrins by purified rat liver plasma membranes.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.