Linked Life Data

7200981

Source:http://linkedlifedata.com/resource/pubmed/id/7200981

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
1982-8-26
pubmed:abstractText
Co-sonicated mixtures of egg phosphatidylcholine and small amounts (less than or equal to 4%, w/w) of [carbonyl-13C]cholesteryl oleate have been studied by 13C NMR spectroscopy at 50.3 MHz. The carbonyl chemical shift from cholesteryl oleate solubilized in vesicles was 1 ppm downfield from the carbonyl chemical shift of cholesteryl oleate present in a separate oil phase. The maximum solubility of the steroid in vesicles determined by chemical analysis of purified vesicles was 1.6 weight % (approximately 2 mol %), in close agreement with the maximum solubility estimated from NMR speak intensity measurements (1.4 weight %). The downfield shift indicates hydrogen bonding of solvent (H2O) molecules with the cholesteryl oleate carbonyl group, suggesting that vesicle-solubilized cholesteryl oleate molecules are located in the phospholipid bilayer with the carbonyl group close to the aqueous interface and the sterol ring and fatty acyl chain approximately parallel to the fatty acyl chains of the phospholipid. Such a folded conformation and localization of the carbonyl group at the aqueous interface may facilitate interactions of sterol esters with cholesterol ester transfer proteins and cholesterol esterases.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
257
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7318-21
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Solubilization and localization of cholesteryl oleate in egg phosphatidylcholine vesicles. A carbon 13 NMR study.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.