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Predicate | Object |
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rdf:type | |
lifeskim:mentions |
umls-concept:C0009630,
umls-concept:C0022702,
umls-concept:C0024264,
umls-concept:C0086418,
umls-concept:C0178602,
umls-concept:C0871261,
umls-concept:C1280500,
umls-concept:C1514485,
umls-concept:C1533691,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C1883712,
umls-concept:C1948023,
umls-concept:C2911692
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pubmed:issue |
1
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pubmed:dateCreated |
1981-10-25
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pubmed:abstractText |
We have examined the effect of alpha-methylmannoside (alpha-MM) addition to concanavalin A (con A)-stimulated peripheral human lymphocytes. With a previously established kinetic model, we have, from the time course of proliferation, extracted the responding clone size, rate of entry of this clone into S phase and the length of the lag period. We have studied the effect of con A dose and time of addition of alpha-MM to optimally stimulated cells on these kinetic parameters. We show that neither a low dose of con A nor an early addition of alpha-MM to optimally stimulated cells results in a change in the responding clone size. This is, all of the potentially cells appears to become "committed" to enter the cell cycle regardless of the presence of alpha-MM early in the culture or in the presence of suboptimal stimulation. However, the rate at which these committed cells enter the first S phase is a function of the dose of con A and time of addition of alpha-MM and varies over a wide range. It is the variation in this parameter that accounts for virtually all of the diminished response previously interpreted as a time-dependent irreversible commitment of mitogen-stimulated cells. The previous work using only fixed time points for measuring thymidine uptake and the concept of commitment must be reevaluated in light of the kinetic evidence presented.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/Mannose,
http://linkedlifedata.com/resource/pubmed/chemical/Methylglycosides,
http://linkedlifedata.com/resource/pubmed/chemical/Methylmannosides,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine,
http://linkedlifedata.com/resource/pubmed/chemical/methylmannoside
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9541
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
108
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
25-34
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7196406-Adult,
pubmed-meshheading:7196406-Cell Cycle,
pubmed-meshheading:7196406-Cells, Cultured,
pubmed-meshheading:7196406-Concanavalin A,
pubmed-meshheading:7196406-Dose-Response Relationship, Drug,
pubmed-meshheading:7196406-Humans,
pubmed-meshheading:7196406-Interphase,
pubmed-meshheading:7196406-Kinetics,
pubmed-meshheading:7196406-Lymphocytes,
pubmed-meshheading:7196406-Mannose,
pubmed-meshheading:7196406-Methylglycosides,
pubmed-meshheading:7196406-Methylmannosides,
pubmed-meshheading:7196406-Middle Aged,
pubmed-meshheading:7196406-Models, Biological,
pubmed-meshheading:7196406-Thymidine
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pubmed:year |
1981
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pubmed:articleTitle |
Commitment and proliferation kinetics of human lymphocytes stimulated in vitro: effects of alpha-MM addition and suboptimal dose on concanavalin A response.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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