Linked Life Data

7170064

Source:http://linkedlifedata.com/resource/pubmed/id/7170064

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1983-6-10
pubmed:abstractText
Following the injection of 4-day old rats with 150 mM L-[3,4-3H]valine (10 mumol/g, IP) the incorporation of 3H into protein was linear 2 hours. Valine specific activity in the brain acid-soluble fraction was constant between 30 and 120 min after injection with a mean value of 82.3% of the injectate. Significant amounts of tritated metabolites accumulated in the brain acid-soluble fraction (41.4% of radioactivity at 120 min) but do not prove an impediment to measuring rates of protein synthesis. The rate of protein synthesis in cerebral cortex of the 4-day old rat was measured by quantitative autoradiography using [3H]valine and 3H-sensitive film. The measured rate shows excellent agreement with that found previously using L-[1-14C]valine. Our results suggest that [3H]valine can be a useful precursor to measure local rates of brain protein synthesis by quantitative autoradiography.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0364-3190
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1507-14
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Measurement of local rates of brain protein synthesis by quantitative autoradiography: validation with L-[3H]valine.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.