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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1983-3-11
|
| pubmed:abstractText |
Histones can be extracted from chicken erythrocyte chromatin with 2 M CaCl2 10 mM Tris (pH 7.4). The core histones so extracted exist as H3-H4 tetramers and H2A-H2B dimers since calcium at greater than 0.5 M result in dissociation of the histone octamer. Reconstitution of octamers occurs on removal of the Ca2+ by dialysis. Although less than 0.5 M calcium do not result in octamer dissociation, perturbations in the structure can be detected by CD and tyrosine fluorescence spectroscopy.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0014-5793
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
29
|
| pubmed:volume |
149
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
277-80
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7152043-Animals,
pubmed-meshheading:7152043-Calcium,
pubmed-meshheading:7152043-Chickens,
pubmed-meshheading:7152043-Circular Dichroism,
pubmed-meshheading:7152043-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7152043-Erythrocytes,
pubmed-meshheading:7152043-Histones,
pubmed-meshheading:7152043-Macromolecular Substances
|
| pubmed:year |
1982
|
| pubmed:articleTitle |
The effect of Ca2+ on the stability of chicken erythrocyte histone octamers.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|