Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1983-1-27
pubmed:abstractText
Characteristic features of collagen metabolism in human skin fibroblasts were studied in relation to cell density. Measuring peptide-bound hydroxyproline we found that collagen synthesis per cell decreased when cultures approached confluency. On the other hand, the relative rate of collagen synthesis (collagen/total protein) was higher in quiescent than in proliferating cultures. With increasing cell density the proportion of type III collagen in comparison with type I was found to be slightly increased. In addition, in low-density cultures [alpha I(I)]3 collagen trimers were produced in considerable amounts, whereas they were no longer detected in cultures with a high cell density. Although hydroxylation of proline residues was normal in all cell stages, conversion of procollagen into collagen was found to depend strongly on the density at which the cells were investigated. Almost no cleavage of procollagen peptides was observed in rapidly growing cells, whereas highly confluent cell cultures converted most of the newly synthesized procollagen molecules.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-1092340, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-1184037, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-1186867, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-14098437, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-16068188, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-171666, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-179471, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-19483, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-206541, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-215834, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-224946, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-225172, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-270689, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-377287, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-428565, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-4342111, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-4349600, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-449904, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-4502941, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-4508318, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-457666, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-4734737, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-4745782, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-5277100, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-5761170, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-5804625, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-5962520, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-6157425, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-621215, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-6243264, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-6265920, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-6266492, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-6269532, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-6765898, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-6932028, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-6999941, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-7015111, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-714169, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-7204395, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-7206599, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-736601, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-7370260, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-7372635, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-7458408, http://linkedlifedata.com/resource/pubmed/commentcorrection/7150259-908458
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
206
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
505-10
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Influence of cell density on collagen biosynthesis in fibroblast cultures.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't