Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2-3
|
pubmed:dateCreated |
1983-1-19
|
pubmed:abstractText |
Following treatment of cultured mosquito cells (Aedes albopictus line of Singh) with the chemical mutagen ethyl methanesulfonate, we were able to isolate three cycloheximide-resistant clones. On the basis of growth kinetics, plating efficiency, and protein synthesis, these clones are 10- to 30-fold more resistant to cycloheximide than the parent cells. Cell-free lysates made from these cells retained 30-65% of their endogenous protein synthesizing ability in the presence of cycloheximide concentrations as high as 300 micrograms/ml. Protein synthesis in lysates from the parental cells, however, is reduced to about 10% of the control value (i.e., in the absence of drug) at 14 micrograms of cycloheximide/ml and was completely abolished at 75 micrograms/ml. These results indicate that cycloheximide resistance in these cells is likely due to an alteration in the protein synthetic machinery. This is the first description of cycloheximide-resistant insect cells, and the best example of cycloheximide resistance in cells originating from a higher eukaryotic organism.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Oct
|
pubmed:issn |
0027-5107
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
96
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
201-12
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:7144797-Aedes,
pubmed-meshheading:7144797-Animals,
pubmed-meshheading:7144797-Cells, Cultured,
pubmed-meshheading:7144797-Clone Cells,
pubmed-meshheading:7144797-Cycloheximide,
pubmed-meshheading:7144797-Drug Resistance,
pubmed-meshheading:7144797-Kinetics,
pubmed-meshheading:7144797-Mutation,
pubmed-meshheading:7144797-Phenotype,
pubmed-meshheading:7144797-Protein Biosynthesis
|
pubmed:year |
1982
|
pubmed:articleTitle |
Isolation and characterization of cycloheximide-resistant mosquito cell clones.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|