Linked Life Data

7140994

Source:http://linkedlifedata.com/resource/pubmed/id/7140994

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
1983-1-7
pubmed:abstractText
Proctolin (H-Arg-Tyr-Leu-Pro-Thr-OH), a highly bioactive peptide found first in insects, is shown to be localized to specific neurons in the central nervous system (CNS) of the cockroach Periplaneta americana. Application of an antiproctolin serum to whole ganglia in a whole-mount immunohistochemical technique reveals the presence of proctolin immunoreactive neurons throughout the CNS. These neurons can be mapped and appear in consistent positions in different individuals. Some have been uniquely identified by intracellular dye injection combined with immunological localization. The presence of proctolin in individually identified neurons has been determined by high-pressure liquid chromatography combined with proctolin bioassay detection performed on extracts made from single identified cells. Some proctolin-containing neurons contain large vacuolelike inclusions in their cell bodies. These vacuoles appear to be the greatly enlarged lumena of the rough endoplasmic reticulum and may be part of the morphological substrate for the manufacture of proctolin. Electrophysiological studies suggest that some proctolin-containing neurons in different segmental ganglia, although not all, are normally recruited together as a functional unit by shared presynaptic input. This characterization of individual proctolin neurons is a prelude to a cellular study of the physiological role of a neuropeptide.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0014-9446
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2940-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Identification of proctolin-containing neurons.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't