Linked Life Data

7118380

Source:http://linkedlifedata.com/resource/pubmed/id/7118380

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1982-12-2
pubmed:abstractText
The thermal denaturation of lysozyme in aqueous sulfoxide solutions (pH 3) was investigated by differential scanning calorimetry (DSC). The sulfoxides employed were dimethylsulfoxide (DMSO), ethylmethylsulfoxide, (DMSO), dimethylsulfoxide (DMSO), and butylmethylsulfoxide (BMSO). The temperature of denaturation, Td, decreased with an increase in the concentration of sulfoxide, the decrease becoming much more pronounced at higher sulfoxide concentrations. The lowering of Td was enhanced by an increase in the hydrocarbon content of the sulfoxide molecule. The enthalpy of denaturation, delta Hd, showed a complex dependence on the solvent composition; the delta Hd first increased with increasing sulfoxide concentration and then started decreasing at different concentrations for each sulfoxide. This behavior is analogous to that with monohydric alcohols, but is different from that with guanidine hydrochloride. These results may be interpreted in terms of the interactions of the sulfoxide added with the protein and with water.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0367-8377
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
25-31
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Effect of dimethylsulfoxide and its homologues on the thermal denaturation of lysozyme as measured by differential scanning calorimetry.
pubmed:publicationType
Journal Article