Linked Life Data

7112915

Source:http://linkedlifedata.com/resource/pubmed/id/7112915

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1982-10-21
pubmed:abstractText
Peripheral blood leucocytes (PBL) isolated 23 times over a 6-week period from four normal sheep showed considerable variation in serially tested responses to predetermined optimal concentrations of concanavalin A (Con A), phytohaemagglutinin (PHA), and pokeweed mitogen (PWM). Con A responses, in particular, varied widely and were often randomly depressed (21 of 91 times compared to 15 of 91 times for PHA or PWM). The addition of as few as 1% adherent cells (AC) to depressed cultures fully restored the PBL proliferative response to normal levels. Addition of greater numbers of AC (5 or 10%) had little further enhancing effect on depressed cultures. The addition of 1, 5, or 10% AC to cultures that were responding at normal levels increased responses only slightly. Autologous or allogeneic AC were equally effective. Addition of 2-mercaptoethanol (2-ME) to depressed cultures only partially restored the blastogenic response to Con A and had little effect on normal cultures.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0165-2427
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
345-59
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Variation in mitogen-induced ovine lymphocyte blastogenesis: adherent cells or 2-mercaptoethanol restore randomly depressed responses.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.