Linked Life Data

7099167

Source:http://linkedlifedata.com/resource/pubmed/id/7099167

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1982-9-10
pubmed:abstractText
Protein carboxymethylase (PCM) activity was evaluated for long-term in vitro cultures of human peripheral blood monocytes and pulmonary alveolar macrophages. Both cell types exhibited increases in endogenous (without addition of the exogenous substrate, gelatin) and total (with gelatin) enzyme activity with increased time in culture. Monocytes developed increased activity after a 5-day lag period; three-to four-fold increases over day 1 values in both total and endogenous specific activity occurred. In contrast, PCM activity increased for pulmonary alveolar macrophage (PAM) without a detectable lag period. Although the increase in endogenous activity of 10--14-day PAM culture was similar to comparable age monocyte cultures, total enzyme activity increased only two-fold above day 1 values. The observation of changes in PCM endogenous specific activity in monocyte cultures may reflect alterations in enzyme activity and/or levels of endogenous methyl-acceptor proteins.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0161-5890
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
281-6
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Protein carboxymethylation during in vitro culture of human peripheral blood monocytes and pulmonary alveolar macrophages.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't