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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1982-8-14
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pubmed:abstractText |
1. A continuous spectrophotometric determination of rat hepatic microsomal anaerobic azo reductase activity has been developed. 2. The addition of soluble flavins (riboflavin, FMN or FAD) greatly increased this NADPH-dependent activity towards a number of azo substrates. 3. Investigations with amaranth as substrate gave an apparent Km of 34 microM and Vmax. of 4 nmol/min per mg of microsomal protein. The inclusion of a fixed concentration of FMN increased Vmax. and greatly decreased Km, the magnitude of these changes reflecting the concentration of flavin present. 4. Investigations using a fixed amaranth concentration over a range of flavin concentrations gave biphasic double-reciprocal plots with two apparent Km and Vmax. values. 5. Pretreatment of animals with cobaltous chloride, 2-allyl-2-isopropylacetamide, carbon tetrachloride, phenobarbitone and 3-methylcholanthrene altered azo reductase activity in parallel with changes in cytochrome P-450 content. 6. The significance of these results is discussed in terms of the electron-transfer components present in the hepatic microsomal fraction.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7092814-13476352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7092814-14007123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7092814-14209971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7092814-15436485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7092814-28474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7092814-4133181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7092814-4341449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7092814-4383667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7092814-4383668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7092814-4400525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7092814-4403481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7092814-5443528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7092814-5500003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7092814-5522537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7092814-923973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7092814-96118
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
201
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
589-95
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7092814-Amaranth Dye,
pubmed-meshheading:7092814-Animals,
pubmed-meshheading:7092814-Azo Compounds,
pubmed-meshheading:7092814-Cytochrome P-450 Enzyme System,
pubmed-meshheading:7092814-Electron Transport,
pubmed-meshheading:7092814-Flavin Mononucleotide,
pubmed-meshheading:7092814-Kinetics,
pubmed-meshheading:7092814-Male,
pubmed-meshheading:7092814-Microsomes, Liver,
pubmed-meshheading:7092814-NADH, NADPH Oxidoreductases,
pubmed-meshheading:7092814-Rats,
pubmed-meshheading:7092814-Rats, Inbred Strains,
pubmed-meshheading:7092814-Spectrophotometry
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pubmed:year |
1982
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pubmed:articleTitle |
A continuous spectrophotometric determination of hepatic microsomal azo reductase activity and its dependence on cytochrome P-450.
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pubmed:publicationType |
Journal Article,
In Vitro
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