Linked Life Data

7088012

Source:http://linkedlifedata.com/resource/pubmed/id/7088012

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1982-8-26
pubmed:abstractText
90 agents have been tested in the HeLa DNA-synthesis inhibition test. This test detects strong mutagens and carcinogens except when difficulties with metabolic activation are encountered. Weaker DNA-damaging agents are generally positive in the test but require relatively high concentrations (greater than 1 mM). Very weak agents, such as saccharin, are not positive. Only 1 false positive has been encountered and the dose response for it (cytochalasin B) is atypical. The test does not seem suitable for complex mixtures, probably because ingredients that do not damage DNA but inhibit DNA synthesis by another mechanism can mask the action of a DNA-damaging agent. Inhibition of DNA synthesis in HeLa and other mammalian cells is best used as a rapid and inexpensive screening procedure to detect relatively strong mutagenic carcinogens.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0027-5107
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
92
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
427-37
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
The Hela DNA-synthesis inhibition test as a rapid screen for mutagenic carcinogens.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.