Linked Life Data

7069793

Source:http://linkedlifedata.com/resource/pubmed/id/7069793

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 1
pubmed:dateCreated
1982-6-21
pubmed:abstractText
A freezing-stage has been developed for use on a standard light-microscope, which can provide reproducible, precisely linear cooling and warming rates in the range from 0.1 to 10,000 K/min. Biological cells in aqueous solutions can be observed during the freeze-thaw cycle; the volume loss due to osmotic efflux of water and the intracellular crystallization of water are detected by video-monitoring. The temperature field generated in the observed samples is comparable to extended cylindrical probes and allows the transfer of cryomicroscopic data to technically used vial geometries. Lymphocytes and granulocytes were observed during freezing using the system described. They were separated and washed, and then frozen on the cold stage of the cryomicroscope at cooling rates ranging from 2 to 500 K/min. Shrinkage of the cells was observed up to 100 K/min and intracellular ice formation could be detected starting at 10 K/min. The results show that human leucocytes show excessive shrinkage up to 36% of their initial volume; the probability of intracellular ice formation exhibits a sharp increase from 10 to 100 K/min where nearly all cells contain ice.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-2720
pubmed:author
pubmed:issnType
Print
pubmed:volume
126
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
29-44
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Thermally defined cryomicroscopy and some applications on human leucocytes.
pubmed:publicationType
Journal Article