Linked Life Data

7045518

Source:http://linkedlifedata.com/resource/pubmed/id/7045518

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1982-8-14
pubmed:abstractText
A procedure for the isolation and long-term in vitro cultivation of endothelial cells from rat cerebral cortical microvasculature is described. Migrating cells emerged from collagenase-treated microvessel fragments as early as 1 to 2 days in culture. Migration continued and marked proliferation began 5 to 7 days after isolation and continued up to 12 to 14 days. Cell colonies developed and consisted of endothelial cells as determined by phase contrast microscopy and cell culture behavior. Proliferation of the endothelial cells was significantly enhanced (3- to 4-fold) with the addition of endothelial cell growth supplement at a concentration of 150 microgram. per ml. Cultures with endothelial cell growth supplement (ECGS) retained their characteristic endothelial morphology for 6 to 8 weeks, after which they exhibited a gradual deterioration and loss of their phenotypic appearance. The endothelial origin of these cells was demonstrated by positive immunofluorescent staining for factor VIII antigen and angiotensin-converting enzyme and lack of binding of rat smooth muscle myosin antibody. Ultrastructural examination of confluent endothelial cell cultures revealed intercellular junctional complexes consisting of gap junctions, punctate fusions, and tight junctions. Since long-term endothelial cell cultures derived from the cerebral microvasculature retain characteristic endothelial cell markers and in vivo markers and in vivo features of brain capillary endothelium, they can serve as a useful model system to characterize microvascular endothelium in a variety of disease states.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0023-6837
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
554-63
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Primary culture of rat cerebral microvascular endothelial cells. Isolation, growth, and characterization.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't