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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1982-8-26
pubmed:abstractText
E colicins are those inactive on btuB mutants, which lack the outer membrane protein that adsorbs vitamin B12, E colicins and phage BF23; types E1, E2, E3 and E7 have been defined by the specific immunity of E-colicinogenic strains to the type of E colicin they produce. Further immunity types - E4, E5, E6 - are now described. Shigella sonnei of colicin type 9 makes colicin E4, and Shigella sonnei of types 9A, 12 and 14 make colicin E6 (not colicin E3, as previously supposed). Many local Shigella sonnei isolates make E colicin of a new type - E5. The action of colicins E1 to E7 was (incompletely) blocked by vitamin B12, which also reduced the effect of A colicins (which are weakly active on Escherichia coli K12 btuB indicators). Escherichia coli K 12 given plasmid Co1A-23 by transformation was immune to A colicins but sensitive to colicins E1 to E7. A purported 'colicin E4' was shown to be of class colicin A. Escherichia coli K 12(CloDF13) transformants were immune to colicin E6. A 'cap' of colicin-sensitive indicator bacteria developed over, but not around, killed colonies of btuB+ E-colicinogenic strains, because of adsorption of colicin by non-induced bacteria of the colony. Killed colonies of btuB+ E-colicinogenic strains gave partly discrete lobes of colicin action on indicator lawns, instead of circular zones, apparently as a result of unstable variation in colicin production. The presence of a colicin E4, E5 or E6 plasmid in K12 btuB+ strains made them more sensitive to colicins E2 and E7, as shown by zones of partial translucency surrounding the ordinary colicin zones and by increased titre of colicin E2 and E7 preparations on such indicators.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-1287
pubmed:author
pubmed:issnType
Print
pubmed:volume
128
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
95-106
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Colicins E4, E5, E6 and A and properties of btuB+ colicinogenic transconjugants.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.