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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
|
pubmed:dateCreated |
1982-5-21
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pubmed:abstractText |
AN enzyme-linked immunosorbent assay (ELISA) and a microtiter agglutination (MA) test were used in a comparative study to measure the humoral antibody responses of turkeys receiving oral vaccination against fowl cholera. The ELISA was sensitive and measured antibody titers as high as 1:4,409, whereas the highest titers the MA test measured were 1:128. A close correlation was seen between ELISA antibody titers and protection against oral challenge with virulent Pasteurella multocida, whereas a poor correlation was seen between antibody titers measured by MA tests and protection. ELISA substrate reactions from a single serum dilution, measured with a spectrophotometer, could be converted directly from absorbance to antibody titers using a linear regression plot.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0005-2086
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
25
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
964-71
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7039594-Agglutination Tests,
pubmed-meshheading:7039594-Animals,
pubmed-meshheading:7039594-Antibodies, Bacterial,
pubmed-meshheading:7039594-Bacterial Vaccines,
pubmed-meshheading:7039594-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:7039594-Immunoenzyme Techniques,
pubmed-meshheading:7039594-Pasteurella,
pubmed-meshheading:7039594-Pasteurella Infections,
pubmed-meshheading:7039594-Poultry Diseases,
pubmed-meshheading:7039594-Turkeys,
pubmed-meshheading:7039594-Vaccination
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pubmed:articleTitle |
Use of an enzyme-linked immunosorbent assay to measure antibody responses in turkeys against Pasteurella multocida.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|