Linked Life Data

7024625

Source:http://linkedlifedata.com/resource/pubmed/id/7024625

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
1981-11-24
pubmed:abstractText
We studied bacterial adherence to uroepithelial cells of female patients in relation to the menstrual cycle. We determined the number of bacteria demonstrable on native cells and the number of adhering bacteria after incubation with E. coli. Free bacteria and uroepithelial cells with adhering bacteria were optimally separated by differential centrifugation using sodium metrizoate (9.6%) and dextran (5.6%) (Lymphoprep). Thus, contamination of the cell preparation by free (non-adhering) bacteria was avoided and results were well reproducible. A median of five bacteria/cell (range 0-19) was observed for uroepithelial cells obtained from urine of female patients. Such bacteria were mostly rods. After in vitro incubation with E. coli cultures, a median of 33 bacteria/cell (range 17-54) was counted. Our improved methodology failed to demonstrate a relationship between bacterial adhesion and the menstrual cycle.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0023-2173
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
59
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
905-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Application of an improved methodology to demonstrate bacterial adherence during the menstrual cycle.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't