Linked Life Data

7024250

Source:http://linkedlifedata.com/resource/pubmed/id/7024250

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1981-11-18
pubmed:abstractText
The lipid A components of substrate cell origin incorporated by Bdellovibrio bacteriovorus during intraperiplasmic growth (D. R. Nelson and S. C. Rittenberg, J. Bacteriol. 147:860-868, 1981) were shown to be integrated into its lipopolysaccharide structure. Lipid A isolated from bdellovibrios grown on Escherichia coli was resolved into two fractions by thin-layer chromatography. Fraction 2 had the same Rf as the single lipid A fraction of axenicaly grown bdellovibrios, and both stained identically with aniline-diphenylamine reagent. Fraction 1 resembled, in Rf and staining reaction, the slower migrating of two lipid A fractions obtained from the E.coli used as the substrate cell. Both fractions 1 and 2 contained glucosamine, a substrate cell-derived compound. Greater than 65% of the fatty acids in fraction 1 were derived from the substrate cell, whereas more than 60% of the fatty acids of fraction 2 were synthesized by the bdellovibrio. Nevertheless, each fraction contained significant amounts of fatty acid of both origins. The substrate cell-derived fatty acids had the same distribution of N-acyl and O-acyl linkages as in E. coli lipid A. The data indicate that the two lipid A moieties in lipopolysaccharide of intraperiplasmically grown bdellovibrios are hybrids of substrate cell-derived and bdellovibrio-synthesized components. The data also suggest that disaccharide units and N- and O-acyl linkages preexisting in the substrate cell lipid A may be conserved. A possible explanation for the unequal distribution of substrate cell-derived material in the two lipid A fractions of the bdellovibrio is suggested.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7024250-13271382, http://linkedlifedata.com/resource/pubmed/commentcorrection/7024250-14155106, http://linkedlifedata.com/resource/pubmed/commentcorrection/7024250-17730453, http://linkedlifedata.com/resource/pubmed/commentcorrection/7024250-221486, http://linkedlifedata.com/resource/pubmed/commentcorrection/7024250-357428, http://linkedlifedata.com/resource/pubmed/commentcorrection/7024250-4908793, http://linkedlifedata.com/resource/pubmed/commentcorrection/7024250-5069711, http://linkedlifedata.com/resource/pubmed/commentcorrection/7024250-5337757, http://linkedlifedata.com/resource/pubmed/commentcorrection/7024250-5804498, http://linkedlifedata.com/resource/pubmed/commentcorrection/7024250-7024249, http://linkedlifedata.com/resource/pubmed/commentcorrection/7024250-809266, http://linkedlifedata.com/resource/pubmed/commentcorrection/7024250-811650
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
147
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
869-74
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Partial characterization of lipid A of intraperiplasmically grown Bdellovibrio bacteriovorus.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't