Linked Life Data

7022177

Source:http://linkedlifedata.com/resource/pubmed/id/7022177

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1981-10-25
pubmed:abstractText
The mutagenic effects of the alkylating agents, MMS and EMS, were studied by measuring the reversion of an arg (ochre) mutation in various DNA-repair-deficient strains. When compared with the wild-type strain, EMS-induced mutagenesis was reduced in the recA13 strain but not in the lexA3 strain. MMS-induced mutagenesis was reduced to background levels in the recA13 strain and reduced to intermediate levels in the lexA3 strain. The umuC36 strain showed intermediate levels of mutagenesis with both mutagens which suggests that a substantial portion of both MMS- and EMS-induced mutagenesis depends upon this component of the error-prone "SOS" repair pathway. The uvrD101, recL152 and recF143 mutations produced increased levels of MMS-induced reversion but had no effect upon the levels of EMS-induced mutagenesis, suggesting that the pathways affected by these genes may play a role in the error-free repair of MMS but not EMS damage. In contrast, a large increase in the level of mutagenesis was noted in a delta uvrB101 mutant with EMS but not MMS. This hypermutability with EMS was also seen in uvrA6, uvrB5 and uvrC34 mutant strains and suggests a role for excision in the error-free repair of ethylation but not methylation damage to DNA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0027-5107
pubmed:author
pubmed:issnType
Print
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
239-50
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Influence of DNA-repair deficiencies on MMS- and EMS-induced mutagenesis in Escherichia coli K-12.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't