Linked Life Data

7020355

Source:http://linkedlifedata.com/resource/pubmed/id/7020355

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-9-15
pubmed:abstractText
Treponema pallidum was discovered relatively late and was not cultured in vitro. Both the delineation of T. pallidum biology and the eradication of syphilis suggest the necessity of cultivation in vitro. An attempt has been made with an improved medium to cultivate pathogenic T. pallidum Budapest strain in vitro. Only in the first passage, evidence of in vitro multiplication of T. pallidum has been established by (i) macroscopic observation, (ii) darkfield examination, (iii) electron microscopic examination, (iv) optical densities, (v) tritium labelled thymidine incorporation, and (vi) the pathogenicity off the cultured organisms was evidenced by rabbit challenge. Explanation of the oxygen utilization of T. pallidum suspension is discussed. Unidentified formations were observed on electron micrographs from the 96 h cultures. They may belong to the multiplication forms of treponemes. Further experiments are needed for their identification and for expansion of the multiplication of T. pallidum beyond the first passage.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0001-6187
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7-24
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Cultivation of pathogenic Treponema pallidum in vitro.
pubmed:publicationType
Journal Article