Linked Life Data

6999778

Source:http://linkedlifedata.com/resource/pubmed/id/6999778

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1980-12-18
pubmed:abstractText
L-cells were infected at high multiplicity with meningopneumonitis organsims (MP) and incubated in medium with penicillin (200 units/ml) and without the drug. At intervals up to 36 hours cells were removed and L-cell associated chlamydial infectivity was determined for cell cultures from the chorioallantoic membrane (CAM) of chick embryos which were treated before inoculation with Diethylaminoethyl (DEAE)-Dextran. In control L-cells, the infectivity decreased at 8 hours and reached it lowest level 16 hours after infection. In penicillin-treated L-cells in contrast, infectivity remained at low levels from 2 and up to 36 hours after infection. At 16 hours after infection about 90% of both penicillin-treated and nontreated L-cells contained single or multiple Giemsa-stained intracytoplasmic inclusions which in both series contained almost exclusively reticulate bodies (RB) by electron microscopy. RB from 16 hour infected penicillin-treated and non-treated L-cells were partially freed from cell debris and their infectivity (ID50) for CAM-culture cells (TC) was assayed 3 times. Penicillin-induced RB had TCID50 of 10(4.0)/ml compared to control RB of 10(4.5)/ml. The TCID50 of the original MP strain for CAM-culture cells was 10(8.0)/ml. Penicillin-induced RB and control RB from 16 hour L-cell samples were frozen and thawed 3 times and titrated in CAM cells; the TCID50 of both series dropped to 10(1.0)/ml while the TCID50 of the original MP inoculum after freezing and thawing 3 times was 10(7.0)/ml.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0172-5599
pubmed:author
pubmed:issnType
Print
pubmed:volume
246
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
119-27
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Infectivity of penicillin-induced Chlamydia psittaci forms for cell cultures from the chorioallantoic membrane of chick embryos.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't