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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5754
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pubmed:dateCreated |
1980-5-23
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pubmed:abstractText |
Double-stranded cDNA prepared from the 12S fraction of poly(A) RNA from interferon (IF)-producing human leukocytes was cloned in Escherichia coli using the pBR322 vector. One of the resulting clones had a 910-base pair insert which could hybridise to IF mRNA and was responsible for the production of a polypeptide with biological IF activity. Up to 10,000 units IF activity per g of cells was obtained from some clones.
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pubmed:language |
eng
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
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|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0028-0836
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pubmed:author |
|
|
pubmed:issnType |
Print
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|
pubmed:day |
27
|
|
pubmed:volume |
284
|
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
|
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pubmed:pagination |
316-20
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pubmed:dateRevised |
2006-5-1
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pubmed:meshHeading |
pubmed-meshheading:6987533-Base Sequence,
pubmed-meshheading:6987533-Cloning, Molecular,
pubmed-meshheading:6987533-DNA, Recombinant,
pubmed-meshheading:6987533-Escherichia coli,
pubmed-meshheading:6987533-Humans,
pubmed-meshheading:6987533-Interferons,
pubmed-meshheading:6987533-Leukocytes,
pubmed-meshheading:6987533-Plasmids,
pubmed-meshheading:6987533-Transformation, Genetic
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|
pubmed:year |
1980
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|
pubmed:articleTitle |
Synthesis in E. coli of a polypeptide with human leukocyte interferon activity.
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pubmed:publicationType |
Journal Article
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