Linked Life Data

6987527

Source:http://linkedlifedata.com/resource/pubmed/id/6987527

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5752
pubmed:dateCreated
1980-5-23
pubmed:abstractText
Origins of replication have been identified in the DNA of viruses, mitochondria, bacterial plasmids and the bacterial chromosome. However, origins of replication of eukaryote chromosomes have remained elusive because of the large size and sequence complexity of chromosomes and in particular for want of a suitable assay for their detection. Recent development of techniques for genetic transformation of yeast by autonomously replicating cytoplasmic plasmids now makes it possible to search for eukaryote origins in a manner analogous to that used for bacteria. Here we describe the construction and properties of a plasmid which contains no effective eukaryote replication origin and whose efficiency of replication in yeast is greatly enhanced by insertion of certain fragments of yeast chromosomal DNA. We believe these to contain replication origins, since yeast transformants are shown to contain copies of the transforming plasmids.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0028-0836
pubmed:author
pubmed:issnType
Print
pubmed:day
13
pubmed:volume
284
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
185-7
pubmed:dateRevised
2006-5-1
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Isolation of chromosomal origins of replication in yeast.
pubmed:publicationType
Journal Article