|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1980-7-22
|
|
pubmed:abstractText |
The protease inhibitor leupeptin inhibits the degradation process of (125)I-labeled epidermal growth factor ((125)I-EGF) by cultured bovine granulosa cells. At 80 mug/ml, leupeptin inhibited the appearance of degradation products of (125)I-EGF in the medium by 95% during 1 hr of incubation and by 90% during 24 hr of incubation when the cells were exposed to 5 ng of (125)I-EGF per ml. In contrast, cultures exposed to either saturating (10 ng/ml) or nonsaturating (0.1 ng/ml) concentrations of EGF in the presence of leupeptin (80 mug/ml) exhibited an increase in DNA synthesis that was 70-80% that of cultures exposed to EGF alone. Cultures responded to either EGF or fibroblast growth factor with a logarithmic increase in cell number and, over a period of 8 days, the number of cells increased 10- to 18-fold. Addition of leupeptin did not diminish the growth rate of the cultures. In the presence of leupeptin, (125)I-EGF accumulated within the granulosa cells and was in a form that was precipitable with antiserum against EGF and that comigrated on isoelectric focusing with native (125)I-EGF. That a full mitogenic response can be obtained despite a 90-95% inhibition of EGF degradation at either saturating or nonsaturating concentrations of the mitogen suggests that a proteolytic degradation of a given mitogen may not be involved in the induction of a proliferative response.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6966398-1012021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6966398-1054489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6966398-14450081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6966398-215996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6966398-220539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6966398-226968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6966398-23215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6966398-300054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6966398-306387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6966398-307248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6966398-307249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6966398-311281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6966398-313931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6966398-4636326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6966398-5012739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6966398-649600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6966398-977646
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
77
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1466-70
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:6966398-Animals,
pubmed-meshheading:6966398-Antipain,
pubmed-meshheading:6966398-Biological Transport,
pubmed-meshheading:6966398-Cattle,
pubmed-meshheading:6966398-Cell Division,
pubmed-meshheading:6966398-Cells, Cultured,
pubmed-meshheading:6966398-DNA Replication,
pubmed-meshheading:6966398-Epidermal Growth Factor,
pubmed-meshheading:6966398-Female,
pubmed-meshheading:6966398-Fibroblasts,
pubmed-meshheading:6966398-Granulosa Cells,
pubmed-meshheading:6966398-Growth Substances,
pubmed-meshheading:6966398-Leupeptins,
pubmed-meshheading:6966398-Mitogens,
pubmed-meshheading:6966398-Peptides,
pubmed-meshheading:6966398-Protease Inhibitors
|
|
pubmed:year |
1980
|
|
pubmed:articleTitle |
Role of the degradation process in the mitogenic effect of epidermal growth factor.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
