rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1982-8-14
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pubmed:abstractText |
A monoclonal antibody of the IgG class was prepared against rat liver glucose-6-phosphate dehydrogenase (G6PD; D-glucose-6-phosphate:NADP+ 1-oxidoreductase, EC 1.1.1.49) by the hybridoma technique. This antibody does not affect the catalytic activity of the enzyme and shows crossreactivity with the palmitoyl CoA-inactivated G6PD. By solid phase radioimmunoassay, the presence of crossreacting materials in comparable amounts was determined in liver homogenate supernatants from rats that had been starved and refed a high-sucrose diet (containing a high level of G6PD activity) and from rats that had been starved and refed a high-fat diet (containing a low level of G6PD activity). These findings indicate that G6PD is present in an inactive form in rats fed a high-fat diet. The monoclonal antibody will facilitate isolation and characterization of the inactive variant G6PD.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-1191279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-13269373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-13520982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-13597742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-13654316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-13823292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-14097352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-14190349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-14488303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-323365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-4153779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-4397678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-4420747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-4622935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-5360673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-5638636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-5834322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-688763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-714156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-7459866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-90677
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
79
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2860-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6953434-Animals,
pubmed-meshheading:6953434-Antibodies, Monoclonal,
pubmed-meshheading:6953434-Antigen-Antibody Reactions,
pubmed-meshheading:6953434-Binding, Competitive,
pubmed-meshheading:6953434-Cross Reactions,
pubmed-meshheading:6953434-Glucosephosphate Dehydrogenase,
pubmed-meshheading:6953434-Liver,
pubmed-meshheading:6953434-Precipitins,
pubmed-meshheading:6953434-Protein Conformation,
pubmed-meshheading:6953434-Rats,
pubmed-meshheading:6953434-Structure-Activity Relationship
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pubmed:year |
1982
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pubmed:articleTitle |
Preparation of a monoclonal antibody to rat liver glucose-6-phosphate dehydrogenase and the study of its immunoreactivity with native and inactivated enzyme.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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