Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1982-8-14
pubmed:abstractText
A monoclonal antibody of the IgG class was prepared against rat liver glucose-6-phosphate dehydrogenase (G6PD; D-glucose-6-phosphate:NADP+ 1-oxidoreductase, EC 1.1.1.49) by the hybridoma technique. This antibody does not affect the catalytic activity of the enzyme and shows crossreactivity with the palmitoyl CoA-inactivated G6PD. By solid phase radioimmunoassay, the presence of crossreacting materials in comparable amounts was determined in liver homogenate supernatants from rats that had been starved and refed a high-sucrose diet (containing a high level of G6PD activity) and from rats that had been starved and refed a high-fat diet (containing a low level of G6PD activity). These findings indicate that G6PD is present in an inactive form in rats fed a high-fat diet. The monoclonal antibody will facilitate isolation and characterization of the inactive variant G6PD.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-1191279, http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-13269373, http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-13520982, http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-13597742, http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-13654316, http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-13823292, http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-14097352, http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-14190349, http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-14488303, http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-323365, http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-4153779, http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-4397678, http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-4420747, http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-4622935, http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-5360673, http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-5638636, http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-5834322, http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-688763, http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-714156, http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-7459866, http://linkedlifedata.com/resource/pubmed/commentcorrection/6953434-90677
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2860-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Preparation of a monoclonal antibody to rat liver glucose-6-phosphate dehydrogenase and the study of its immunoreactivity with native and inactivated enzyme.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.