Linked Life Data

6952937

Source:http://linkedlifedata.com/resource/pubmed/id/6952937

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1982-8-26
pubmed:abstractText
Interactions involved in the binding of the anthracycline analogues adriamycin, carminomycin, pyrromycin, musettamycin, marcellomycin, and aclacinomycin to calf thymus DNA and covalently closed circular PM-2 DNA have been studied. Fluorescence quenching experiments revealed that denaturation of calf thymus DNA and increasing ionic strength each resulted in a marked decrease in the DNA binding affinities of all of the anthracyclines studied. These results suggest that intercalative and electrostatic interactions are both important in the DNA binding of these analogues. Viscometric studies indicated that under high ionic strength conditions which negated electrostatic effects, all of the anthracyclines induced an unwinding--rewinding process of the closed superhelical PM-2 DNA typically observed for DNA intercalators. Relative to the 26 degrees unwinding angle of ethidium bromide, anthracyclines with a daunomycinone-like aglycon induced an unwinding angle of approximately 13 degrees. This differed slightly from the unwinding angles of 10.3 degrees-11.1 degrees which were induced by anthracyclines with a pyrromycinone-like aglycon. Increases in the length of the glycosidic side chain did not elicit significant differences in PM-2 DNA unwinding ability, implying a lack of effect of glycosidic side chain length on the anthracycline intercalation process. The unwinding angles also showed little sensitivity to decreases in ionic strength, suggesting that the fraction of bound anthracycline molecules which are in a nonintercalated state is similar to the fraction of bound ethidium bromide molecules which are in a nonintercalated state.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1541-7
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Viscometric and fluorometric studies of deoxyribonucleic acid interactions of several new anthracyclines.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't