Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1982-7-22
pubmed:abstractText
High-affinity 125I-labelled monoclonal antibodies to hepatitis B virus surface antigen (HBsAg) bind to human hepatocellular carcinoma cell line, PLC/PRF/5, which synthesizes and secretes HBsAg. These monoclonal antibodies of the IgG1, IgG2a, and IgM isotypes are directed against different antigenic determinants on HBsAg and, in the presence of complement, both anti-HBs IgG2a and IgM, but not anti-HBs IgG1, lyse PLC/PRF/5 cells in culture. Although there is a low level of anti-HBs binding (especially with anti-HBs IgM) to human hepatoma cell lines which do not synthesize HBsAg (SK-Hep 1 and Mahlavu cells), this interaction does not lead to complement-mediated cell lysis and is thought to be nonspecific. Minimal binding of 125I-labeled anti-influenza HA antigen IgM binding to PLC/PRF/5 cells was also detected, but this likewise did not lead to complement-mediated cell lysis. These results indicate that a human hepatocellular carcinoma cell line, persistently infected with hepatitis B virus, can be recognized and lysed by monoclonal antibodies directed against specific determinants of HBsAg. Monoclonal antibodies to this viral envelope protein may prove to be useful immunodiagnostic and immunotherapeutic agents when such viral epitopes are expressed on the surface of infected cells.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-212785, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-221611, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-233137, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-286328, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-316541, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-499332, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-59675, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-6161061, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-6161957, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-6166367, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-6166691, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-6248094, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-6249525, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-6250073, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-6258074, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-6260336, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-6267609, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-6268980, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-6269974, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-6450241, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-6940137, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-710851, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-7228971, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-7286902, http://linkedlifedata.com/resource/pubmed/commentcorrection/6952217-833871
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
650-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Selecting binding and complement-mediated lysis of human hepatoma cells (PLC/PRF/5) in culture by monoclonal antibodies to hepatitis B surface antigen.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't