Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1982-3-22
pubmed:abstractText
Phenotypically nontoxinogenic mutants of Vibrio cholerae were isolated after infection with either of two mutagenic vibriophages, VcAI and VcA2ctsl. DNA isolated from these mutants was analyzed for toxin gene sequences by the Southern blotting method with 32P-labeled probes derived from the cloned A and B subunit genes for the heat-labile enterotoxin of Escherichia coli, designated LT. Several of the mutant isolates were shown by this method to have lost all sequences hybridizing to the LT probes, indicating that these clones contain deletion mutations that removed the structural gene(s) for cholera toxin. The mutants were prototrophic and grew normally, in vitro, demonstrating that the toxin is not essential for the growth and viability of V. cholerae. Moreover, the toxin gene deletion mutants multiplied well in vivo in ligated rabbit intestine. Because of these growth properties and the stability of deletion mutations, these strains are promising candidates for testing as live oral vaccine strains for protection against cholera.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-1055375, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-1091563, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-109838, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-1114331, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-16558096, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-16789156, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-208069, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-210449, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-228285, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-3214, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-345281, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-383697, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-4129825, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-4443613, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-447068, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-4560760, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-4654645, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-4731194, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-479110, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-4891264, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-4927022, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-580785, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-6156914, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-6260754, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-669812, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-6998963, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-7251142, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-730346, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-7364714, http://linkedlifedata.com/resource/pubmed/commentcorrection/6948297-797313
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
151-5
pubmed:dateRevised
2010-9-10
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Isolation of enterotoxin structural gene deletion mutations in Vibrio cholerae induced by two mutagenic vibriophages.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't