Linked Life Data

6945297

Source:http://linkedlifedata.com/resource/pubmed/id/6945297

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1981-12-21
pubmed:abstractText
A human myeloid cell line, HL-60, has been shown to terminally differentiate to mature granulocytes in the presence of dimethylsulfoxide (DMSO) and other compounds. A histone polypeptide (HP) with an apparent molecular weight of 12,500 has been identified among the acid-extractable chromosomal proteins of DMSO-treated HL-60 cells. The detection of HP in total chromatin, total cell proteins or acid extracts of intact HL-60 cells treated with DMSO indicates that HP is present in intact cells and is not generated during the isolation procedures used for preparation of chromatin or histones. No radiolabelled HP was detected when chromatin was isolated from a mixture of radiolabelled mouse cells and HL-60 cells treated with DMSO. The relative increase in the amount of HP in DMSO-treated HL-60 cells correlates with a concomitant decrease of histone H2A. Fingerprint analysis of tryptic digests shows HP to be related to histone H2A. Generation of HP from endogenous and exogenous H2A. Generation of HP from endogenous and exogenous H2A is probably due to a proteolytic enzyme associated with the chromation of the differentiated HL-60 cells. HP was not observed in chromatin from a number of human or mouse hematopoietic tissues and cell lines but low levels of HP were detected in human lymphocytes and granulocytes prepared from peripheral blood of normal donors. HP was also detected in leukocytes from patients with acute leukemia. Our results suggest that: (1) HP is a proteolytic product of histone H2A and not a product of an independent gene; (2) the observed level of HP in HL-60 cells in related to the stage of differentiation; and (3) HP is a potential biological marker for human acute leukemia.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0020-7136
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
585-92
pubmed:dateRevised
2007-7-24
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Detection of the histone-2A related polypeptide in differentiated human myeloid cells (HL-60) and its distribution in human acute leukemia.
pubmed:publicationType
Journal Article