Linked Life Data

6933266

Source:http://linkedlifedata.com/resource/pubmed/id/6933266

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1981-1-29
pubmed:abstractText
The effects of prospidine, chemically known as 3, 12-diaza-6,9 diazoniadispiro[5.2.5.2]hexadecane,3,12-bis(3-chloro-2-hydroxypropyl)-dichloride (NSC-166100), on cell viability, growth, and colony formation were investigated in several mammalian cell lines. Cell cycle progression and the terminal point of action of the drug were monitored by flow cytometry. Prospidine was cytostatic for two suspension cultures (Friend leukemia and L1210 cells) at a concentration of 10 mg/ml during the first cell cycle after exposure to the drug. Cells were blocked in G2 at lower concentrations of prospidine (e.g., 1.0 mg/ml) but only after 12--24 hours of continuous exposure, i.e., during the second cell cycle in the presence of drug. Cells could be observed to accumulate in G2 by 24 hours even if prospidine (0.1 mg/ml) was removed after 12 hours. A short incubation with a liver cytosol fraction (30 min) or with cultured cells (12 hr) failed to enhance the potency of the drug. Formation of colonies of the adherent Chinese hamster ovary cell line was inhibited by 50% following 24-hour exposure to 1.1 mg prospidine/ml. Under culture conditions in which cells were blocked in G2, their RNA content increased only slightly, but the incorporation of [5-(3)H]uridine into RNA was suppressed by 15--20%. Incubation of cells with prospidine increased the stability of DNA in situ to acid-induced denaturation, which thus suggested that the drug may interact with cellular DNA.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0027-8874
pubmed:author
pubmed:issnType
Print
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
993-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Effects of prospidine on survival and growth of mammalian cells in culture.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.