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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1981-1-29
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pubmed:abstractText |
The sera of some patients with SLE contain an IgG antibody (F-42) directed against the classical pathway C3 convertase (C-42), which is capable of stabilizing C42 in a dose-dependent manner. The half-life (T 1/2) of C42 is prolonged by F-42. In order to determine whether C4-binding protein was capable of reversing stabilization of C42, stabilized and unstabilized cell-bound C42 were exposed to purified C4-bp and the convertase activity was assessed. C4-bp was capable of accelerating the decay of C42 in a dose-dependent manner; 2 microgram/ml C4-bp reduced the T 1/2 of C42 from 5 to 2.5 min at 30 degrees C. On the other hand, 16 microgram C4-bp could reverse stabilization of C42 by F-42 from T 1/2 = 78 min to a T 1/2 - 40 min; 128 microgram C4-bp reduced the T 1/2 of stabilized C42 to 4 min. Functional inactivation of C42 occurs via enhanced decay-dissociation of C2 from the convertase by C4-bp, as shown by the release of 125I-C2i from the cell-bound convertase. Stabilization of C42 by F-42 is caused by prevention of decay-dissociation of 125I-C2. F-42 was also capable of stabilizing C4oxy2 even further, as shown by prolongation of the T 1/2 of cell-bound C4 oxy2 to a T 1/2 of at least 300 min at 30 degrees C.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Complement Activating Enzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Complement C2,
http://linkedlifedata.com/resource/pubmed/chemical/Complement C3-C5 Convertases,
http://linkedlifedata.com/resource/pubmed/chemical/Complement C4
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
125
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2051-4
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:6903579-Antibodies,
pubmed-meshheading:6903579-Carrier Proteins,
pubmed-meshheading:6903579-Complement Activating Enzymes,
pubmed-meshheading:6903579-Complement Activation,
pubmed-meshheading:6903579-Complement C2,
pubmed-meshheading:6903579-Complement C3-C5 Convertases,
pubmed-meshheading:6903579-Complement C4,
pubmed-meshheading:6903579-Complement Pathway, Classical,
pubmed-meshheading:6903579-Dose-Response Relationship, Immunologic,
pubmed-meshheading:6903579-Humans
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pubmed:year |
1980
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pubmed:articleTitle |
Relative resistance of the F-42-stabilized classical pathway C3 convertase to inactivation by C4-binding protein.
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pubmed:publicationType |
Journal Article
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