Linked Life Data

6863281

Source:http://linkedlifedata.com/resource/pubmed/id/6863281

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
1983-8-17
pubmed:abstractText
A plasma membrane-associated serine esterase (protease) has previously been reported to be required during stimulation of a variety of cell types. In this study we have examined the role of such a protease on membrane potential changes using an optical probe of membrane potential 3,3'-dipropylthiodicarbocyanine iodide. The irreversible chymotrypsin-like protease inhibitor TPCK (tosyl-L-phenylalanyl chloromethyl ketone) inhibited membrane potential changes in rat neutrophils in response to phorbol myristate acetate, N-formylmethionylleucylphenylalanine and the calcium ionophore A23187 in a time- and dose-dependent manner. This inhibition was correlated with the known inhibitory effects on superoxide (O-2) generation and enzyme release (lysozyme and beta-glucuronidase). High external calcium concentrations overcame the inhibitory effects of TPCK on A23187-induced stimulation but had no effect on the inhibition of N-formylmethionylleucylphenylalanine stimulation. These results suggest that a TPCK-inhibitable activity is required for the development of the membrane potential changes that are coupled to subsequent secretory events and precede a calcium requiring activity.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
258
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8123-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Inhibition by tosyl-L-phenylalanyl chloromethyl ketone of membrane potential changes in rat neutrophils. Correlation with the inhibition of biological activity.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.