Linked Life Data

6833771

Source:http://linkedlifedata.com/resource/pubmed/id/6833771

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1983-5-27
pubmed:abstractText
A new method for monocyte isolation based on cell adherence to gelatin-coated plastic and dislodgement of the adhering monocytes at low temperature was compared with 5 other methods based on cell adherence to substrate and density gradient separation. All methods produced yields of monocytes ranging approximately between 50-70% with about the same degree of purity (less than 90%) except for the method using Percoll density gradient centrifugation where the purity of monocytes was about 80%. When lidocaine at different concentrations was used for cell dislodgement or Percoll density gradient for separation, phagocytosis, Fc receptor function and cytotoxicity were adversely affected, unlike in methods using EDTA or low temperature for dislodgement of the adherent cells. In monocyte chemotaxis assays the rate of migration was affected but not the number of migrating cells for all the isolation procedures investigated. Cell spreading function was apparently well maintained only when gelatin coated plastic was used for adherence and low temperature for cell dislodgement. These data indicate that the newly described method, similar to methods using EDTA for cell dislodgement, yielded relatively intact monocytes but unlike the latter method with better preserved cell spreading. Thus, this method can be considered for standardization to obtain pure monocyte populations from peripheral blood which then can be submitted for comprehensive biochemical and physiologic studies.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
309-21
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Evaluation of methods using adherence to substrate and density gradient for the isolation of human monocytes.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.