Linked Life Data

6815785

Source:http://linkedlifedata.com/resource/pubmed/id/6815785

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1983-1-19
pubmed:abstractText
Several of many tested plant lectins induced interferon (IFN) production in cultures of human peripheral blood mononuclear leucocytes (PBL). The mannose-binding lectin obtained from Lens culinaris (LCL) was a particularly efficient inducer of trypsin-sensitive antiviral activity, which qualified as IFN-gamma because it was 90-95% destroyed by pH 2 treatment but not neutralized by anti-IFN-gamma antibodies. However, such antibodies neutralized the residual 5-10% pH 2-resistant IFN, which therefore represented IFN-alpha. Further evidence for the IFN-gamma nature of the LCL-induced IFN was that its production in PBL cultures required both T lymphocytes and macrophages and that its induction of antiviral resistance in human amnion cells was significantly delayed compared with IFN-alpha. Under optimal conditions LCL induced titres of IFN-gamma corresponding to more than 20,000 IFN-alpha units/ml medium, higher than observed with other tested, established IFN-gamma inducers. Other desirable properties of this lectin, as discussed, also suggest that it will be of value for efficient large-scale IFN-gamma production.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0300-9475
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
327-31
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Lectins as inducers of interferon-gamma production in human lymphocytes: lentil lectin is highly efficient.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro